Fig. 6: PHDMSC-CM down-regulates inflammatory responses in rats with NEC at systemic and musocal level. | Cell Death & Disease

Fig. 6: PHDMSC-CM down-regulates inflammatory responses in rats with NEC at systemic and musocal level.

From: Prolyl hydroxylase 2 silencing enhances the paracrine effects of mesenchymal stem cells on necrotizing enterocolitis in an NF-κB-dependent mechanism

Fig. 6

a (Upper panel) Representative images of the cytokine antibody array are shown. The rectangles highlight cytokines significantly different between NEC rats receiving PHDMSC-CM and NEC rats receiving MSC-CM (fold change > 2.0). Each sample consisted of a pool of serum from five different donor rats. Each measurement was duplicated reproducibly. (Lower panel) The graphs show the relative intensity fold changes of the 27 cytokines detected by antibody-based protein array. b The same serum samples were assayed by ELISA for six selected cytokines. **P < 0.05 vs. NEC + DMEM-F12. ##P < 0.05 vs. NEC + MSC-CM. c The levels of pro/anti-inflammatory cytokines in intestinal protein extracts on day 4 of life were determined by ELISA. n = 4 rats/group. **P < 0.05 vs. NEC + DMEM-F12. ##P < 0.05 vs. NEC + MSC-CM. d The percentages of CD4+Foxp3+ Treg cells in the CD4+ population of mesenteric lymph nodes (MLNs) were determined by flow cytometry (4–5 rats/group). Tissue samples were collected on day 4 of life. **P < 0.05 vs. NEC + DMEM-F12. ##P < 0.05 vs. NEC + MSC-CM.

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