Fig. 7: Localized electroporation loading of cells within the bystander zone with cell-impermeable inhibitors of Ca2+ and ROS signaling pathways strongly reduce γ-H2AX scores. | Cell Death & Disease

Fig. 7: Localized electroporation loading of cells within the bystander zone with cell-impermeable inhibitors of Ca2+ and ROS signaling pathways strongly reduce γ-H2AX scores.

From: Cx43 channels and signaling via IP3/Ca2+, ATP, and ROS/NO propagate radiation-induced DNA damage to non-irradiated brain microvascular endothelial cells

Fig. 7

a Schematic overview of the electroporation experiment, with indication of the irradiated zone and electroporation zone located in the bystander area. b Fluorescence image of the electroporation zone in RBE4 cells as visualized by 10 kDa dextran Texas red (DTR) immediately after electroporation (0 h time point; size bar 50 µm). c Experiment as in b but loaded with fura red as a diffusion reporter (0 h time point). d Full width at half maximum (FWHM) for both dyes at 0 h and later. e Representative γ-H2AX images with indication of the electroporation zone loaded with vehicle or BAPTA (size bar 1 mm). Dotted line indicates the border of the irradiated area. f Summary γ-H2AX data in the electroporation zone, demonstrating that SOD, BAPTA, and BH4-Bcl2 significantly decreased γ-H2AX counts compared with vehicle. An asterisk (*) vs. vehicle (n = 4–5).

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