Fig. 4: Ablation of endogenous SBDS inhibits cancer cell growth and invasion.
From: Dual regulation of p53 by the ribosome maturation factor SBDS
a, b Knockdown of SBDS by siRNA inhibits proliferation of HCT116p53+/+ cells more dramatically than that of HCT116p53−/− cells. Cells were transfected with control or SBDS siRNA and re-seeded in 96-well plate followed by the cell viability assay. c, d Knockdown of SBDS by shRNAs inhibits proliferation of HCT116p53+/+ cells more dramatically than that of HCT116p53−/− cells. Cells were infected with control or SBDS-shRNA lentiviruses and re-seeded in 96-well plate followed by the cell viability assay. e, f Knockdown of SBDS induces sub-G1 accumulation of HCT116p53+/+ cells but not HCT116p53−/− cells. Cells were transfected with control or SBDS siRNA for 48–72 hr followed by flow cytometry analyses. g, h Knockdown of SBDS prevents invasion of HCT116p53+/+ cells more dramatically than that of HCT116p53−/− cells. Cells were transfected with control or SBDS siRNA followed by the transwell cell invasion assay.
