Fig. 5: Ectopic SBDS induces p53 stabilization and activation.
From: Dual regulation of p53 by the ribosome maturation factor SBDS
a Ectopic expression of SBDS induces the expression of p53 and its target gene p21 in H460 cells. Cells were transfected with the empty vector or SBDS plasmid followed by IB analysis. b Ectopic SBDS induces the expression of p53 and its target gene p21 in a dose-dependent manner in HCT116p53+/+ cells. Cells were transfected with increased dosage of SBDS plasmid and followed by IB analysis. c Lentivirus-delivered ectopic SBDS induces the expression of p53 and its target genes, MDM2 and p21. HCT116p53+/+ cells were infected with control or SBDS-expressing lentiviruses followed by IB analysis. d, e Ectopic SBDS prolongs p53 protein half-life. HCT116p53+/+ cells were transfected with the empty vector or SBDS plasmid for 30–48 hr and treated with CHX for the indicated time followed by IB analysis. The ratios of p53 to GAPDH were presented in e. f Ectopic SBDS counteracts MDM2-induced p53 degradation. HCT116p53+/+ cells were transfected with combinations of Flag-SBDS, HA-MDM2, and empty vector followed by IB analysis. g Ectopic SBDS impairs MDM2-induced p53 ubiquitination. H1299 cells were transfected with combinations of p53, His-Ub, HA-MDM2, and Flag-SBDS followed by in vivo ubiquitination assay and IB analysis. Poly-ubiquitination of p53 was indicated by the open curly brace.
