Fig. 4: CircHIPK3 and LncGAS5 interact directly with miR-495 and regulate its expression. | Cell Death & Disease

Fig. 4: CircHIPK3 and LncGAS5 interact directly with miR-495 and regulate its expression.

From: The regulatory network among CircHIPK3, LncGAS5, and miR-495 promotes Th2 differentiation in allergic rhinitis

Fig. 4

a The predicted binding sequences and the artificially mutated sequences between CircHIPK3/LncGAS5 and miR-495. b The relative luciferase activity after the co-transfection with miR-495 mimic and the CircHIPK3/LncGAS5 mutant/wild type (MUT/WT) reporter vector. c The relative miR-495 level in the complex which was pulled down by CircHIPK3 or LncGAS5 probe using the RAP assay. d Relative expression of CircHIPK3 and LncGAS5 in the complex which was pulled down by biotin-labeled miR-495 using the RNA pull-down assay. e The cellular distribution of CircHIPK3, LncGAS5, and miR-495 in OVA and Th2 differentiation-induced CD4+ T cells using the FISH assay. f Relative expression of LncGAS5 and miR-495 after CircHIPK3 knockdown or overexpression. g Relative expression of CircHIPK3 and miR-495 after LncGAS5 knockdown or overexpression. **p < 0.01 vs negative controls (pre-NC, Biotin NC, or NC). ##p < 0.01 vs lentivirus control (Lenti-GFP).

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