Fig. 5: CircHIPK3 and LncGAS5 increase GATA-3 expression via downregulating miR-495.

a The predicted binding sequences and the artificially mutated sequences between miR-495 and the 3′-untranslated region (UTR) of GATA-3. b The relative luciferase activity after the co-transfection with miR-495 mimic and the GATA-3 3′-UTR mutant/wild type (MUT/WT) reporter vector. c The relative luciferase activity after the co-transfection with miR-495 inhibitor and the GATA-3 3′-UTR mutant/wild type (MUT/WT) reporter vector. d CD4⁺ T cells were transfected with Lenti-CircHIPK3/Lenti-LncGAS5 or co-transfected with miR-495 mimic followed by the induction of OVA and Th2 differentiation. The GATA-3 protein level was detected using western blot analysis. e CD4⁺ T cells were also transfected with si-CircHIPK3/si-LncGAS5 or co-transfected with miR-495 inhibitor followed by the induction of OVA and Th2 differentiation. The GATA-3 protein level was detected using western blot analysis. **p < 0.01 vs negative controls (pre-NC or NC).