Fig. 2: Top2α inhibition induces CD4 T cell apoptosis and dysfunction. | Cell Death & Disease

Fig. 2: Top2α inhibition induces CD4 T cell apoptosis and dysfunction.

From: Inhibition of topoisomerase IIA (Top2α) induces telomeric DNA damage and T cell dysfunction during chronic viral infection

Fig. 2

a Top2α levels in CD4 T cells treated with DMSO (con) or Top2α inhibitor (ICRF-193) in the presence of anti-CD3/CD28 stimulation for 6, 24, 48, and 72 h, were determined by immunoblotting. b Top2α protein levels in CD4 T cells that were treated with DMSO (Cont) or Top2α inhibitor (ETP) with TCR (anti-CD3/CD28) stimulation for 6, 24, and 48 h. c Top2cc levels in genomic DNA from CD4 T cells that were exposed to DMSO control or Top2α inhibitor (ICRF or ETP) for 72 h, were measured by immunoblotting. d Representative dot plots and summary data of IL-2 and IFN-γ expression in TCR-stimulated CD4 T cells that were exposed to ETP or DMSO control. e T cell proliferation, measured by CFSE dilution, in TCR-stimulated CD4 T cells in the presence of ICRF and DMSO control for 5 d. f Av and 7-AAD staining of CD4 T cells that were treated with various concentrations of ICRF-193 for 1, 2, 3, and 5 days, determined by flow cytometry. g The vulnerability of CD4 T cells that were derived from viral infected individuals and HS to the ICRF-193-induced apoptosis, were determined by flow cytometry.

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