Fig. 6: PCDHGA9 inhibits EMT and metastasis via β-catenin redistribution in GC cells. | Cell Death & Disease

Fig. 6: PCDHGA9 inhibits EMT and metastasis via β-catenin redistribution in GC cells.

From: PCDHGA9 represses epithelial-mesenchymal transition and metastatic potential in gastric cancer cells by reducing β-catenin transcriptional activity

Fig. 6

a MKN-28 and SGC-7901 cells with stable overexpression or knockdown of PCDHGA9 were transfected with or without β-catenin, and the indicated protein expression was assessed. b MKN-45 cells with stable PCDHGA9 knockdown were treated with XAV-939 (10 μmol/l) for 24 h, and SGC-7901 cells with stable PCDHGA9 overexpression were treated with CHIR-99021 (10 μmol/l) for 24 h. Then, the TOP/FOP luciferase activity assay was employed, and c the indicated proteins were assessed via western blotting. SGC-7901, MKN-28 and MKN-45 cells with stable overexpression or knockdown of PCDHGA9 were treated with CHIR-99021 (10 μmol/l) or XAV-939 (10 μmol/l) for 24 h as indicated and subjected to a d wound healing assay, e invasion assay and f migration assay. The data are presented as the mean ± SEM of three independent experiments. **p < 0.01, ***p < 0.001 by two-tailed Student’s t-test.

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