Fig. 7: PCDHGA9 interacts with β-catenin and antagonizes the Wnt pathway to attenuate TGF-β-induced EMT. | Cell Death & Disease

Fig. 7: PCDHGA9 interacts with β-catenin and antagonizes the Wnt pathway to attenuate TGF-β-induced EMT.

From: PCDHGA9 represses epithelial-mesenchymal transition and metastatic potential in gastric cancer cells by reducing β-catenin transcriptional activity

Fig. 7

a Nuclear translocation of β-catenin and EMT were suppressed by PCDHGA9 overexpression or XAV-939 treatment, as detected by western blotting. Cells were treated with 10 ng/ml TGF-β1 for 48 h. b The positive effects of TGF-β1 on migration and invasion were suppressed by PCDHGA9 overexpression or XAV-939 treatment. c Immunoprecipitation of β-catenin with PCDHGA9 in PCDHGA9-overexpressing SGC-7901 and negative control cells as detected by western blot analysis. Cells were treated with 10 ng/ml TGF-β1 for 48 h. d TOP/FOP luciferase activity results suggested that β-catenin/TCF4 transcription activity was activated after TGF-β1 treatment, whereas it was obviously inhibited by PCDHGA9 overexpression. The data are presented as the mean ± SEM of three independent experiments.

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