Fig. 3: Large lipid platform (LLP) formation is elevated in CAV1(−) EC cells independently of stress induction. | Cell Death & Disease

Fig. 3: Large lipid platform (LLP) formation is elevated in CAV1(−) EC cells independently of stress induction.

From: Caveolin-1 regulates the ASMase/ceramide-mediated radiation response of endothelial cells in the context of tumor–stroma interactions

Fig. 3

a Large lipid platform formation in CAV1(+) and CAV1(−) EC was visualized with Cholera-toxin B-subunit (purple) that binds ganglioside GM1 after irradiation with 0 (control) or 10 Gy. The lentiviral-transduced EC expresses GFP (green) as a reporter gene and nuclei were stained with DAPI (blue). Representative images (10 min after irradiation) of three individual experiments are shown (photographs). Quantification of LLP formation was done by counting positive cells of control-irradiated (0 Gy) and irradiated (10 Gy) cells. The graph shows LLP formation 10 and 30 min after IR. In total, at least 50 cells/condition were quantified (n = 3, SEM). Statistical analysis was performed with two-way ANOVA followed by post hoc Tukey’s test (****p < 0.001). b In addition, co-staining of LLP (purple) and CAV1 (red) of GPF-labeled (green) CAV(+) EC upon 10-Gy irradiation was performed. Arrows point to CAV1-immunoreactive structures, whereas asterisks mark EC with lower CAV1 immunoreactivity. Representative images are shown. Nuclei were stained with DAPI (blue). Scale bar: 10 µm.

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