Fig. 4: Direct interaction of Cav1-deficient EC with PCa cells increased RT-induced growth retardation. | Cell Death & Disease

Fig. 4: Direct interaction of Cav1-deficient EC with PCa cells increased RT-induced growth retardation.

From: Caveolin-1 regulates the ASMase/ceramide-mediated radiation response of endothelial cells in the context of tumor–stroma interactions

Fig. 4

a LNCaP and b PC3 cells were co-cultured with CAV1(+) or CAV1(−) EC in hanging drops for 24 h. After formation of spheroids, cells were plated in growth factor-reduced Matrigel mixed with normal growth medium (1:2, v/v) and irradiated at 10 Gy. Pictures were taken at the time of irradiation (0 h) and 48 h later. Scale bar represents 50 µm. Representative phase-contrast images from three individual experiments are shown. Spheroid growth was measured and the respective volumes were calculated. Graphs depict the measurements from three independent experiments (n = 3, SEM) where at least ten spheroids per condition each were measured. *p < 0.05, **p < 0.01, ****p < 0.001 by two-way ANOVA followed by post hoc Tukey’s test. Cell death was analyzed afterward by fluorescence microscopy using propidium iodide. DAPI was used for nuclei staining. Representative phase-contrast images and simultaneously recorded fluorescent photographs from three individual experiments are shown (48 h’ time point). Scale bar represents 25 µm.

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