Fig. 5: Exogenous ceramide treatment induced PCa cell growth retardation and cell death. | Cell Death & Disease

Fig. 5: Exogenous ceramide treatment induced PCa cell growth retardation and cell death.

From: Caveolin-1 regulates the ASMase/ceramide-mediated radiation response of endothelial cells in the context of tumor–stroma interactions

Fig. 5: Exogenous ceramide treatment induced PCa cell growth retardation and cell death.The alternative text for this image may have been generated using AI.

a LNCaP and b PC3 cells were cultured as spheroids and irradiated with 0 (control), 2, or 10 Gy in the presence of C6- and C16 ceramide (Cer) or vehicle control (in normal growth media, NGM). Pictures were taken at the time of irradiation (0 h) and 48 h later. Scale bar represents 50 µm. Representative phase-contrast images from three individual experiments are shown. Graphs depict spheroid volumes as determined from three independent experiments (n = 3, SEM) where at least 10–15 spheroids per condition each were measured. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001 (compared with 0-Gy NGM); #p < 0.05, ##p < 0.01, ###p < 0.005 (compared with 2-Gy NGM); +p < 0.05, ++p < 0.01 (compared with 10-Gy NGM) by two-way ANOVA followed by post hoc Tukey’s test. Cell death was analyzed afterward by fluorescence microscopy using propidium iodide. Representative phase-contrast (pc) images and simultaneously recorded fluorescent photographs (0 and 10 Gy; 48-h time point) from three individual experiments are shown. Scale bar represents 25 µm.

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