Fig. 3: Alteronol induces autophagy through Akt/mTOR pathway.

a A375 and UACC62 cells treated with increased concentrations (0, 0.5, 1, 2 μM) of alteronol. Immunoblotting was performed with antibodies as indicated. b A375 and UACC62 cells treated with/without alteronol in the presence or absence of LY294002 as indicated. Immunoblotting assays were performed to measure the phosphorylation status of Akt and mTOR and the accumulation of LC3. All data are presented as the mean ± S.D. for three independent experiments, *p < 0.05, **p < 0.01.