Fig. 4: Alteronol-induced autophagy attenuates apoptosis. | Cell Death & Disease

Fig. 4: Alteronol-induced autophagy attenuates apoptosis.

From: Autophagy inhibition potentiates the anti-EMT effects of alteronol through TGF-β/Smad3 signaling in melanoma cells

Fig. 4

a–c A375 and UACC62 cells were treated with or without alteronol in the presence or absence of 3-MA. a caspase 3 and LC3 were measured by immunoblotting. b The percentage of apoptotic cell was evaluated by flow cytometry. c The cell viability was evaluated by MTT. All the data were expressed as the mean ± S.D. of triplicate experiments. *p < 0.05, **p < 0.01. d, e A375 and UACC62 cells with or without Beclin1 knockout in the presence or absence of alteronolt as indicated. d caspase 3 and LC3 were analyzed by immunoblotting. e The cell viability was evaluated by MTT. The data were expressed as the mean ± S.D. of triplicate experiments. *p < 0.05, **p < 0.01.

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