Fig. 2: Hyperosmotic stress renders exclusive BCL-XL inhibition sufficient for cell death induction. | Cell Death & Disease

Fig. 2: Hyperosmotic stress renders exclusive BCL-XL inhibition sufficient for cell death induction.

From: NOXA-dependent contextual synthetic lethality of BCL-XL inhibition and “osmotic reprogramming” in colorectal cancer

Fig. 2: Hyperosmotic stress renders exclusive BCL-XL inhibition sufficient for cell death induction.

a–d Cells were challenged with the indicated concentrations of WEHI-539, A1155463, A1331852 and S63845 for 18 h in the presence and absence of the indicated concentrations of NaCl. e HCT116 cells were challenged with WEHI-539 (0.6 µM) and NaCl (60 mM) for 4 h and subsequently analyzed by flow cytometry for 7-AAD- and annexin-V positivity. f HCT116 cells were treated with the BCL-XL inhibitors WEHI-539, A1155463 and A1331852 in the presence and absence of NaCl (60 mM). Caspase-3/-7 activity was assessed using the fluorogenic substrate (DEVD)2-R110. g BAX/BAK-deficient variants of HCT116, DLD1 and SW48 cells were challenged with the indicated concentrations of A1155463 and A1331852 (both BCL-XL selective) for 18 h in the presence and absence of the indicated concentrations of NaCl. For (a–d, f and g), data points and mean ± SEM from three independent experiments are shown; for (e), data shown are representative of two experiments performed. RFU relative fluorescence units.

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