Fig. 3: Carnosol inhibits the assembly of inflammasome complexes but has no effect on mitochondrial damage. | Cell Death & Disease

Fig. 3: Carnosol inhibits the assembly of inflammasome complexes but has no effect on mitochondrial damage.

From: Carnosol inhibits inflammasome activation by directly targeting HSP90 to treat inflammasome-mediated diseases

Fig. 3: Carnosol inhibits the assembly of inflammasome complexes but has no effect on mitochondrial damage.

a Western blot analysis of ASC oligomerization in cell lysates of LPS-primed BMDMs treated with various doses of carnosol and then stimulated with nigericin. b Western blot analysis of ASC oligomerization in cell lysates of LPS-primed BMDMs treated with carnosol (10 μM) and then stimulated with ATP, nigericin, poly(I:C), SiO2 or in cell lysates of Pam3CSK4-primed BMDMs treated with carnosol (10 μM) and then stimulated with LPS. c Percentage of ROS-positive cells in LPS-primed BMDMs with carnosol and then stimulated with or without ATP, followed by staining with MitoSox. d Confocal microscopy analysis in LPS-primed BMDMs treated with carnosol and then stimulated with nigericin for 30 min, followed by staining with MitoTracker Red. Scale bars represent 5 µm. e Qualification of potassium efflux in LPS-primed BMDMs treated with various doses of carnosol and then stimulated with nigericin. Data are represented as the mean ± SD from at least four biological samples. The significance of the differences was analyzed using Mann–Whitney U test: *P < 0.05, **P < 0.01, ***P < 0.001 vs. the control, NS: not significant.

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