Fig. 4: Carnosol inhibits inflammasome activation by directly targeting HSP90 and then inhibits its ATPase activity. | Cell Death & Disease

Fig. 4: Carnosol inhibits inflammasome activation by directly targeting HSP90 and then inhibits its ATPase activity.

From: Carnosol inhibits inflammasome activation by directly targeting HSP90 to treat inflammasome-mediated diseases

Fig. 4: Carnosol inhibits inflammasome activation by directly targeting HSP90 and then inhibits its ATPase activity.

a Cell lysates of LPS-primed BMDMs stimulated with or without nigericin. Cells were incubated with sepharose-carnosol for 12 h and proteins pulled down with sepharose beads. b Cell lysates of LPS-primed BMDMs. Cells were incubated with sepharose-carnosol and carnosol for 12 h and proteins pulled down with sepharose beads. c Effect of carnosol on the Activity of HSP90 ATPase. After incubation with HSP90 plus DMSO, HSP90 plus carnosol (0.5 mM, 1 mM), or HSP90 plus GA (30 μM), ATP was measured by Cell Titer Glo. d Activity of caspase-1 in Sup. from LPS-primed BMDMs treated with carnosol (10 μM) or GA (10 μM) and then stimulated with nigericin, poly(dA:dT) and Salmonella or from Pam3CSK4-primed BMDMs treated with carnosol (10 μM) or GA (10 μM) and then stimulated with LPS. Data are represented as the mean ± SD from at least four biological samples. The significance of the differences was analyzed using Mann–Whitney U test: *P < 0.05, **P < 0.01, ***P < 0.001 vs. the control, NS: not significant.

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