Fig. 6: Carnosol suppresses liver injury and fibrosis in an experimental NASH model. | Cell Death & Disease

Fig. 6: Carnosol suppresses liver injury and fibrosis in an experimental NASH model.

From: Carnosol inhibits inflammasome activation by directly targeting HSP90 to treat inflammasome-mediated diseases

Fig. 6: Carnosol suppresses liver injury and fibrosis in an experimental NASH model.

a–h Eight-week-old male C57BL/6 mice were placed on a methionine- and choline-deficient (MCD) diet or an identical diet supplemented with methionine and choline (MCS) for 6 weeks. The mice were treated with carnosol (20 mg/kg) or MCC950 (20 mg/kg) every day for 5 days and then treated with carnosol (40 mg/kg) or MCC950 (40 mg/kg) every 2 days for up to 6 weeks. a Macroscopic appearance of the liver. b, c Serum levels of ALT (b) and AST (c). d Representative micrographs of liver H&E staining, Masson staining, and Sirius red staining. Scale bars represent 100 µm. e, f ELISA of IL-1β (e) and TNF-α (f). g The levels of pro caspase-1, cleaved caspase-1, α-SMA and ASC proteins in the liver were determined by western blotting. Data are represented as the mean ± SD. The significance of the differences was analyzed using Mann–Whitney U test: *P < 0.05, **P < 0.01, ***P < 0.001 vs. the control, NS, not significant.

Back to article page