Fig. 4: The effect of BECN1 on CRC metastasis depends on STAT3. | Cell Death & Disease

Fig. 4: The effect of BECN1 on CRC metastasis depends on STAT3.

From: The autophagy-independent role of BECN1 in colorectal cancer metastasis through regulating STAT3 signaling pathway activation

Fig. 4

a Western blotting assay showing the expression of proteins in the indicated cells. GAPDH was used as the loading control. b, c Cell migration and invasion assays in LoVo and HCT116 cells expressing negative control shRNA, shRNA-BECN1, as well as in those cells transfected with si-STAT3 or treated with CPT. The right panel shows the histograms of the results. The values are the mean ± SD for triplicate samples (*P < 0.05 by an independent Student’s t test). Scale bar: 100 μm. d Wound-healing assays in LoVo and HCT116 cells expressing control negative shRNA, shRNA-BECN1#1, or shRNA-BECN1#2, as well as in those transfected with si-STAT3 or shRNA-BECN1, after treatment with CPT. The right panel shows the histograms of the results. The values are the mean ± SD for triplicate samples (*P < 0.05 by an independent Student’s t test). Scale bar: 200 μm.

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