Fig. 3: Circadian rhythm growth phenotype of HCT116 cells treated with IL6 and IL8.
From: Effect of naive and cancer-educated fibroblasts on colon cancer cell circadian growth rhythm
a ProtoArray data suggesting downregulated IL6 and IL8 expression in TAFs compared to their expression in naive fibroblasts. Positive control dot intensities are shown on the left. b The mRNA expression levels of IL6 and IL8 in naive fibroblasts and in TAFs. c The mRNA expression analysis of IL6 and IL8 from normal colon, benign adenomas, and adenocarcinomas isolated from the same patient. d The mRNA expression analysis of IL6 in naive fibroblasts and in TAF. e The mRNA expression analysis of IL6R in naive fibroblasts and in TAF. f The mRNA expression analysis of IL8 in naive fibroblasts and in TAF. g The mRNA expression analysis of IL8R in naive fibroblasts and in TAF. h The mRNA expression analysis of IL6 in HCT116 cells cultured alone and at the presence of HIF. i The mRNA expression analysis of IL6R in HCT116 cells cultured alone and at the presence of HIF. j The mRNA expression analysis of IL8 in HCT116 cells cultured alone and at the presence of HIF. k The mRNA expression analysis of IL8R in HCT116 cells cultured alone and at the presence of HIF. l DNA replication analysis of the HCT116 cells treated with IL6 demonstrated dampened circadian rhythm amplitude, suggesting a lack of significant differences in cell proliferation during the 44-h follow-up, whereas the growth for the control HCT116 cells was significantly different at time points between 32 and 38 h (p < 0.05) and between 38 and 44 h (p < 0.05). m The incubation of HCT116 cells with IL8 significantly enhanced time-dependent DNA replication during the 20–44 h follow-up period. n Harmonic regression analysis curve of the HCT116 control cells and the IL6-treated cells. The analysis is based on the DNA replication results. o Harmonic regression analysis curve of the HCT116 control cells and the IL8-treated cells. The analysis is based on the DNA replication results. p Harmonic regression analysis parameters period, amplitude, and acrophase for the IL6 and IL8 treatments. The p-values are *p < 0.05, **p < 0.01, and ***p < 0.001.
