Fig. 5: IL6 has early and late effects on HCT116 cell growth.
From: Effect of naive and cancer-educated fibroblasts on colon cancer cell circadian growth rhythm
a The growth curve of IL6-treated HCT116 cells shows significant growth inhibition at the early phase 24 h (p < 0.001) and 48 h (p < 0.01) time points and significantly increased cell numbers at the 72 h (p < 0.05) and 96 h (p < 0.001) time points. b The results from the mRNA analysis of the differentiation marker CEA in the IL6-treated HCT116 cells show significantly (p < 0.001) increased expression. c The differentiation marker CDX2 mRNA analysis in the IL6-treated HCT116 cells shows significantly (p < 0.001) increased expression. d The results from the Western blot analysis of ERK1/2 and ERK5 phosphorylation in IL6-treated HCT116 cells demonstrated no differences in ERK1/2 activation status but significantly decreased ERK5 phosphorylation. e, f. Intensity analysis of ERK1/2 and ERK5 phosphorylation. The p-values are *p < 0.05, **p < 0.01, and ***p < 0.001.
