Fig. 5: Neddylation blockade by MLN4924 prevents HFD-induced hepatic steatosis of mice. | Cell Death & Disease

Fig. 5: Neddylation blockade by MLN4924 prevents HFD-induced hepatic steatosis of mice.

From: Neddylation of sterol regulatory element-binding protein 1c is a potential therapeutic target for nonalcoholic fatty liver treatment

Fig. 5: Neddylation blockade by MLN4924 prevents HFD-induced hepatic steatosis of mice.The alternative text for this image may have been generated using AI.

a−e Mice were divided into four groups (n = 9 per group): vehicle treatment of NCD-fed mice or HFD-fed mice, MLN4924 treatment (30 mg/kg) of NCD-fed mice or HFD-fed mice. Mice were fed HFD for 12 weeks, and MLN4924 was injected intraperitoneally twice weekly for 12 weeks. a Schematic diagram of in vivo model. b Representative images showing the morphology (top), H&E staining (middle) and Oil O Red staining (bottom) of liver sections from HFD-fed mice (n = 9 images per group). c Mice were weighed for 12 weeks. d Liver weight measured after 12 weeks (left). e Hepatic triglyceride levels were measured in extracted liver tissues (right). The data are presented as the means ± SD (n = 9 per group). *P < 0.05 versus control group. f The expression of SREBP1c in liver tissues was analyzed by immunohistochemistry using anti-SREBP1c antibody and IgG used as a negative control (n = 9 per group). Representative images of immunohistochemistry were captured in liver tissues with ×400 magnification. g Western blots for the expression of SREBP1c in the livers of four groups of mice. The band intensities of SREBP1c protein were calculated using ImageJ and plotted by graph (mean ± SD, n = 3). h Total RNAs was extracted from liver tissues of four groups of mice. SREBP1c, FASN, SCD1, ACC, and AGPAT mRNA levels were quantified by RT-qPCR. Results were quantified as relative levels vs. 18S RNA level. All data were presented as the mean ± SD (n = 9 per group).

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