Fig. 1: MiR-4476 is overexpressed in glioma and promotes glioma cell proliferation, migration, and invasion in vitro. | Cell Death & Disease

Fig. 1: MiR-4476 is overexpressed in glioma and promotes glioma cell proliferation, migration, and invasion in vitro.

From: MicroRNA-4476 promotes glioma progression through a miR-4476/APC/β-catenin/c-Jun positive feedback loop

Fig. 1: MiR-4476 is overexpressed in glioma and promotes glioma cell proliferation, migration, and invasion in vitro.

a qPCR analysis of miR-4476 expression in nontumor brain tissues (NB) and glioma tissues. b The cell viability of U87 and LN229 cells transfected with miR-4476 mimics or inhibitor was evaluated by the MTT assays. c The proliferation ability of U87 and LN229 cells was evaluated by EdU assays; scale bar = 50 μm. d, e The migration ability and invasion ability of U87 and LN229 cells were tested by Transwell (d) and Boyden (e) assays, respectively; scale bar = 75 μm. f, g Western blot analysis examined the expression of crucial cell-cycle (f) and EMT (g) related proteins. Mean ± S.D., *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

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