Fig. 3: MiR-4476 directly targets APC and activates Wnt/β-catenin signaling pathway. | Cell Death & Disease

Fig. 3: MiR-4476 directly targets APC and activates Wnt/β-catenin signaling pathway.

From: MicroRNA-4476 promotes glioma progression through a miR-4476/APC/β-catenin/c-Jun positive feedback loop

Fig. 3: MiR-4476 directly targets APC and activates Wnt/β-catenin signaling pathway.

a miR-4476 and its putative binding sequences in the 3’UTR of APC. A mutation was generated in the complementary site that binds to the seed region of miR-4476. b Western blot demonstrated downregulated APC level under miR-4476 mimics transfection, whereas co-transfection with miR-4476 inhibitor reversed this effect. c APC mRNA expression was detected by QPCR in miR-4476-overexpressing U87 and LN229 cells. d APC expression was evaluated by IHC in xenografts derived from U87 cells; scale bar = 75 μm. e Luciferase reporter assay was used to determine miR-4476 direct targeting the APC 3’UTR. f TOPFlash/FOPFlash reporter assay was used to investigate the influence of miR-4476 in Wnt/β-catenin signaling. Mean ± S.D., *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. wt, wild-type; mut, mutant.

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