Fig. 4: Effect of 3OC12HSL on the ubiquitination of PON2 in HeLa cells. | Cell Death & Disease

Fig. 4: Effect of 3OC12HSL on the ubiquitination of PON2 in HeLa cells.

From: WTAP and BIRC3 are involved in the posttranscriptional mechanisms that impact on the expression and activity of the human lactonase PON2

Fig. 4

a Western blot with anti-PON2 showing the protein behavior in HeLa cells induced with 100 μM 3OC12HSL for the indicated times. GAPDH and PARP-1 fragmentation are also reported b IP with anti-Ubiquitin and detection with anti-PON2 of band d after 20 min incubation with 3OC12HSL c Densitometric analysis for quantization of band d. Data were reported as the average of two or three experiments or single data points as shown.

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