Fig. 5: Role of PON2 polymorphisms A148G and S311C. | Cell Death & Disease

Fig. 5: Role of PON2 polymorphisms A148G and S311C.

From: WTAP and BIRC3 are involved in the posttranscriptional mechanisms that impact on the expression and activity of the human lactonase PON2

Fig. 5

a Coomassie-stained gel of recombinant purified PON2 (wild-type), its polymorphic mutants A148G and S311C, and the deletion mutant 123-134del rPON2 produced in E. coli; M: molecular markers. b Representative gel filtration analysis of rPON2 and 123-134del rPON2 mutants. c, d SAXS-based reconstruction of the PON2 wt and 123-134del rPON2 structures. The ab initio rigid models for PON2 c and 123-134del rPON2 d. The structures shown in the figure were drawn at the same spatial scale to compare them. The semi-transparent envelopes in c represent the low resolution models of PON2, where the homology atomic structure model has been docked.

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