Fig. 6: Silencing of the WTAP and WDR36. | Cell Death & Disease

Fig. 6: Silencing of the WTAP and WDR36.

From: WTAP and BIRC3 are involved in the posttranscriptional mechanisms that impact on the expression and activity of the human lactonase PON2

Fig. 6

Expression analysis by quantitative real-time analyses of genes belonging to the “PON2 Cluster” upon the silencing for 24 h of WTAP in the HeLa a and A549 b cell lines. Expression analysis of the four E3UbLs and the two RBPs belonging to the “PON2 Cluster” upon the silencing for 48 h, respectively, of WTAP c, WDR36 d and the co-silencing of WTAP and WDR36 e in the HeLa cell line. f Western blot analysis to determine PON2 expression in WTAP depleted HeLa cells. No-targeting siRNAs (NT) were used as negative control. Total lysate from NT-siRNAs and WTAP siRNAs were electrophoresed and detected with anti-PON2 antibody. g Same experiment as in f but after treatment of HeLa cells with proteasome inhibitor MG132. Data were reported as the average of three or four independent experiments or single values as shown. Please note that in this experiment the ratio between bands b and c is slightly different from what observed in Fig. 1b with a predominance of c (likely due to extraction or cell culturing conditions).

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