Fig. 7: BIRC3 affects PON2 expression level but not its ubiquitination. | Cell Death & Disease

Fig. 7: BIRC3 affects PON2 expression level but not its ubiquitination.

From: WTAP and BIRC3 are involved in the posttranscriptional mechanisms that impact on the expression and activity of the human lactonase PON2

Fig. 7

a Expression analysis by quantitative real-time analyses of indicated genes for 48 h in HeLa cell line. Data from single experiments and average are shown as indicated. Western Blot analysis was made to determine PON2 expression and ubiquitination level in BIRC3-depleted HeLa cells. NT-siRNAs were used as negative control. Total lysates from NT-siRNA and BIRC3 siRNA cells were electrophoresed and detected with anti-PON2 antibody b and anti-ubiquitin antibody d. c Densitometric analysis of bands b and c from three different experiments. Data from single experiments and average are shown as indicated. e Western blot analysis to determine CASP3 activation in BIRC3-depleted HeLa cells. NT-siRNAs were used as negative control. Total lysates from NT siRNAs and BIRC3 siRNAs-treated cells were electrophoresed and detected with anti-CASP3 antibody. In both lanes is only present the pro-enzyme at 32 kDa in its inactive form. No bands is detected at 17 kDa. f Western blot analysis to determine CASP3-mediated PARP-1 cleavage in BIRC3-depleted HeLa cells. NT-siRNAs were used as negative control. Total lysates from NT-siRNAs and BIRC3 siRNAs treated cells were electrophoresed and detected with anti-PARP antibody. Data show only the presence of the enzyme at 113 kDa. No increase is detected for the 89 kDa band.

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