Fig. 4: Evidence for an ATP-dependent increase in orotate.
From: Formate induces a metabolic switch in nucleotide and energy metabolism
a–c Changes in AICAR, orotate and ATP following supplementation of 50 μM of purine metabolites to SHMT2 (T2) deficient and WT cells. d Scatter plot of orotate versus ATP. e Schematic model of orotate flux balance. f Fit of the theoretical model (line) to the scatter plot of orotate versus ATP. Notations: Symbols represent independent experiments. Error bars represent the standard deviation. Solid bars indicate significant change relative to untreated cells of the same genetic background (p < 0.05, two-sided, unequal variance, T test).
