Fig. 1: Role of CHPF in MM tissues and cells. | Cell Death & Disease

Fig. 1: Role of CHPF in MM tissues and cells.

From: Chondroitin polymerizing factor (CHPF) promotes development of malignant melanoma through regulation of CDK1

Fig. 1

a The expression of CHPF in MM tissues and adjacent normal skin tissues were detected by IHC analysis (Magnification ×400). b The upregulated expression of CHPF in MM tissues was proved through data mining of GSE3189 and GSE15605 datasets. c, d The efficiencies of CHPF knockdown in A375 and OM431 cells were detected by qPCR and Western blot analysis, respectively. e MTT assay was performed to detect the cell proliferation rate of A375 and OM431 cells transfected with shCtrl or shCHPF. f Colony formation assay was utilized to compare the colony formation ability of MM cells transfected with shCtrl or shCHPF. g Flow cytometry analysis was performed to show the apoptotic cell percentage in A375 and OM431 cells with or without CHPF knockdown. The data were expressed as mean ± SD (n ≥ 3), *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page