Fig. 7: CDK1 knockdown alleviates the effects of CHPF over-expression on MM cells. | Cell Death & Disease

Fig. 7: CDK1 knockdown alleviates the effects of CHPF over-expression on MM cells.

From: Chondroitin polymerizing factor (CHPF) promotes development of malignant melanoma through regulation of CDK1

Fig. 7

The expression levels of CHPF and CDK1 in A375 cells with simultaneously CHPF over-expression and CDK1 knockdown were detected by qPCR (a) and WB (b), respectively. The influence of CDK1 knockdown on the CHPF over-expression induced changes of proliferation, colony formation, migration and apoptosis of A375 cells was detected by Celigo cell counting assay (c), colony formation assay (d), wound-healing assay (e), Transwell assay (f) and flow cytometry (g), respectively. h, i The in vivo experiments were also performed to reveal the influence of CDK1 knockdown on the CHPF over-expression induced promotion of tumor growth. All data were collected from at least three independent experiments and were normalized to corresponding negative control for facilitating comparison. The figures are representative data from at least three independent experiments. The data were expressed as mean ± SD (n ≥ 3), *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page