Fig. 3: Knock down of SVEP1 promotes HCC cell migration, invasion, and proliferation in vitro. | Cell Death & Disease

Fig. 3: Knock down of SVEP1 promotes HCC cell migration, invasion, and proliferation in vitro.

From: The novel miR-1269b-regulated protein SVEP1 induces hepatocellular carcinoma proliferation and metastasis likely through the PI3K/Akt pathway

Fig. 3: Knock down of SVEP1 promotes HCC cell migration, invasion, and proliferation in vitro.

a WB showing the expression of SVEP1 at the protein level in 7 HCC cell lines (Hep3B, PLC, MHCC97L, MHCC97H, and MHCCLM3 are hepatitis B positive, Huh7 and HLE are hepatitis B negative). b The construction of the SVEP1 control group and SVEP1 knockdown Hep3B HCC cell line. c GSEA of the relationship between the expression of SVEP1 and tumor metastasis. d High-throughput microscopy comparing the migration ability of the SCR and SVEP1/KD groups. e Comparison of the chemotaxis potential the SVEP1/KD and SCR groups. f The invasive ability of the SVEP1/KD and SCR groups in the invasion assay. g Wound-healing assay comparing the translocation distance between the SVEP1/KD and SCR groups. h GSEA of the correlation between the expression level of SVEP1 and tumor proliferation. i CCK-8 assay showing the proliferation ability of the SVEP1/KD and SCR groups.

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