Fig. 2: Nestin regulated the expression and phosphorylation of nephrin in MPCs stimulated with LN plasma.
From: Nestin protects podocyte from injury in lupus nephritis by mitophagy and oxidative stress
a, b Expression of nestin, nephrin and p-nephrin (Y1217) protein in renal cortex of MRL/lpr mice was detected by Western blot. *P < 0.05 vs. MRL/MPJ mice, #P < 0.05 vs. MRL/lpr-MP mice (n = 3). c, d Western blot assay showed the nestin, nephrin and p-nephrin (Y1217) expression in MPCs cultured with 10% LN plasma. *P < 0.05 vs. 0 h group (n = 3). e, f Western blot assay showed the nestin, nephrin and p-nephrin (Y1217) expression in MPCs, which knocked down nestin expression and treated with 10% LN plasma for 24 h. *P < 0.05 vs. control plasma group, #P < 0.05 vs. LN plasma + shNC group (n = 3). g, h Western blot assay showed the nestin, nephrin and p-nephrin (Y1217) expression in MPCs, which knocked down nestin expression. *P < 0.05 vs. shNC group (n = 3). i, j Western blot assay showed the nestin expression in MPCs after transfected with the WT-Nestin plasmid. *P < 0.05 vs. NC group (n = 3). k, l Western blot assay showed the nestin, nephrin and p-nephrin (Y1217) expression in MPCs, which was transfected with WT-Nestin plasmid and treated with 10% LN plasma for 24 h. *P < 0.05 vs. control plasma group, #P < 0.05 vs. LN plasma + NC group (n = 3). m, n Western blot assay showed the nestin, nephrin and p-nephrin (Y1217) expression in MPCs, which upregulated nestin expression by transfection with WT-Nestin plasmid. *P < 0.05 vs. NC group (n = 3). Bonferroni’s correction was performed to analyze statistical significance. Values are mean ± SEM.
