Fig. 6: Nestin could regulate interacted ROS production and mitophagy caused by LN plasma. | Cell Death & Disease

Fig. 6: Nestin could regulate interacted ROS production and mitophagy caused by LN plasma.

From: Nestin protects podocyte from injury in lupus nephritis by mitophagy and oxidative stress

Fig. 6: Nestin could regulate interacted ROS production and mitophagy caused by LN plasma.The alternative text for this image may have been generated using AI.

a, b MitoSOX and FCM assay showed the ROS production of MPCs exposed to LN plasma. Scale bars: 25 μm. c Quantification of DCFH-DA mean fluorescence intensity showing changes in fluorescence intensity in MPCs exposed to LN plasma in different times. DCFH-DA fluorescence was normalized with the mean fluorescence intensity of 0 h group. *P < 0.05 vs. 0 h group (n = 3). d, e Western blot assay showed the expression of nestin, PINK1, LC3 and p62 in the MPCs, which were pretreated with NAC and exposed to LN plasma for 24 h. *P < 0.05 vs. control plasma group, #P < 0.05 vs. LN plasma + DMSO group, n.s. no significance (n = 3). f, g Western blot assay showed the expression of nestin, PINK1, LC3 and p62 in the MPCs, which were pretreated with Mito-TEMPO and exposed to LN plasma for 24 h. *P < 0.05 vs. control plasma group, #P < 0.05 vs. LN plasma + DMSO group, n.s. no significance (n = 3). h, i MitoSOX and FCM assay showed the ROS production of MPCs, which were pretreated with 3-MA and exposed to LN plasma for 24 h. Scale bars: 25 μm. j Quantification of DCFH-DA mean fluorescence intensity showing changes in fluorescence intensity in MPCs exposed to different treatment. DCFH-DA fluorescence was normalized with the mean fluorescence intensity of control plasma group. *P < 0.05 vs. control plasma group, #P < 0.05 vs. LN plasma group (n = 3). k, l MitoSOX staining and FCM showed the upregulation and downregulation of nestin affected the production of ROS in the MPCs exposed to LN plasma for 24 h. Scale bars: 25 μm. m Quantification of DCFH-DA mean fluorescence intensity showing changes in fluorescence intensity in MPCs exposed to different treatments. DCFH-DA fluorescence was normalized with the mean fluorescence intensity of the control plasma group. *P < 0.05 vs. control plasma group, #P < 0.05 vs. LN plasma + shNC group, △P < 0.05 vs. LN plasma + NC group (n = 3). Bonferroni’s correction was performed to analyze statistical significance. Values are mean ± SEM.

Back to article page