Fig. 7: Abnormal ROS production induced the PTP1B/Fyn imbalance and promoted phosphorylation of nephrin. | Cell Death & Disease

Fig. 7: Abnormal ROS production induced the PTP1B/Fyn imbalance and promoted phosphorylation of nephrin.

From: Nestin protects podocyte from injury in lupus nephritis by mitophagy and oxidative stress

Fig. 7

a, b Western blot assay showed the expression of PTP1B and fyn in the MPCs exposed to LN plasma. *P < 0.05 vs. 0 h group (n = 3). c–e Western blot assay showed the expression of PTP1B, nephrin and p-nephrin (Y1217) in the MPCs, which were pretreated with PTP1B inhibitor to inhibit the expression of PTP1B and exposed to LN plasma for 24 h. *P < 0.05 vs. control plasma group, #P < 0.05 vs. LN plasma + DMSO group (n = 3). f–h Western blot assay showed the expression of fyn, nephrin and p-nephrin (Y1217) in the MPCs, which were pretreated with SU6656 to inhibit the expression of fyn and exposed to LN plasma for 24 h. *P < 0.05 vs. control plasma group, #P < 0.05 vs. LN plasma + DMSO group (n = 3). i, j Western blot assay showed the expression of PTP1B, nephrin and p-nephrin (Y1217) in the MPCs, which were pretreated with NAC and exposed to LN plasma for 24 h. *P < 0.05 vs. control plasma group, #P < 0.05 vs. LN plasma + DMSO group (n = 3). k, l Western blot assay showed the expression of PTP1B, nephrin and p-nephrin (Y1217) in the MPCs, which were pretreated with Mito-TEMPO and exposed to LN plasma for 24 h. *P < 0.05 vs. control plasma group, #P < 0.05 vs. LN plasma + DMSO group (n = 3). m, n Western blot assay showed the expression of PTP1B in the MPCs, which were pretreated with 3-MA to inhibit the autophagy and exposed to LN plasma for 24 h. *P < 0.05 vs. control plasma group, #P < 0.05 vs. LN plasma group (n = 3). o, p Knockdown of the expression of PINK1 effectively by siPINK1 in MPCs exposed to LN plasma for 24 h, Western blot assay showed the expression of PTP1B. *P < 0.05 vs. control plasma group, #P < 0.05 vs. LN plasma + siNC group (n = 3). q, r Western blot assay showed the downregulation of nestin affected the expression of PTP1B and fyn in the MPCs exposed to LN plasma for 24 h. *P < 0.05 vs. control plasma group, #P < 0.05 vs. LN plasma + shNC group (n = 3). s, t Western blot assay showed the downregulation of nestin affected the expression of PTP1B and fyn in the MPCs. *P < 0.05 vs. control FBS group (n = 3). Bonferroni’s correction was performed to analyze statistical significance. Values are mean ± SEM.

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