Fig. 1: Drug effectiveness in liver cancer cells cultured in spheroids.

Effect of Sorafenib, Regorafenib, Lenvatinib, and Cabozantinib in the area of spheroids generated by HepG2 (a), Hep3B (b), and Huh7 (c) cells. Drugs (10µM) were administered at day 8th after spheroid establishment, and cultures were maintained up to day 15th as described in “Materials and methods” section. The area of the spheroids (µm2, %, fold over control) were measured at days 8th, 10th, 12th, and 15th. All results are expressed as mean±SD of independent experiments (n = 3). The groups with statistically significant differences among them (p ≤ 0.05) were indicated with different letters (a, b, c, d, e, or f). Magnification of images are ×10.