Fig. 3: Drug effectiveness in liver cancer cells cultured in monolayer. | Cell Death & Disease

Fig. 3: Drug effectiveness in liver cancer cells cultured in monolayer.

From: Differential effectiveness of tyrosine kinase inhibitors in 2D/3D culture according to cell differentiation, p53 status and mitochondrial respiration in liver cancer cells

Fig. 3

Effect of Sorafenib, Regorafenib, Lenvatinib, and Cabozantinib in BrdU incorporation (a) and caspase-3 activity (b) in HepG2, Hep3b, and Huh7 cells cultured in 2D system. Drugs (10µM) were administered at 24h after plating. BrdU incorporation and caspase-3 activity were determined 24h after drug administration using commercial colorimetric assay and caspase-Glo® Assay Systems as described in “Materials and methods” section, respectively. BrdU incorporation is shown as the absorbance at 370nm (reference wavelength: 492nm; absorbance, %, fold over control). Caspase-3 activity is shown as the RLU (%, fold over control). All results are expressed as mean ± SD of independent experiments (n = 4). The groups with statistically significant differences among them (p ≤ 0.05) were indicated with different letters (a, b, c, d, e, f, or g).

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