Fig. 4: Drug effectiveness on cell proliferation in liver cancer cells cultured in monolayer. | Cell Death & Disease

Fig. 4: Drug effectiveness on cell proliferation in liver cancer cells cultured in monolayer.

From: Differential effectiveness of tyrosine kinase inhibitors in 2D/3D culture according to cell differentiation, p53 status and mitochondrial respiration in liver cancer cells

Fig. 4

Effect of Sorafenib (a), Regorafenib (b), Lenvatinib (c), and Cabozantinib (d) in BrdU incorporation in HepG2, Hep3b, Huh7, SNU423, SNU449, and primary human hepatocytes cultured in 2D system. Graphs are separated according to treatments. Drugs (0, 100nM, 1µM, 10µM, and 100µM) were administered at 24h after plating. BrdU was determined 24h after drug administration using a commercial colorimetric assay, as described in as described in “Materials and methods” section. BrdU incorporation is shown as the absorbance at 370nm (reference wavelength: 492nm; absorbance, %, fold over control). All results are expressed as mean±SD of independent experiments (n = 6). The groups with statistically significant differences among them (p ≤ 0.05) were indicated with different letters (a, b, c, d, or e).

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