Fig. 6: Drug effectiveness on cell proliferation receptor protein expression in HepG2 cells cultured in monolayer. | Cell Death & Disease

Fig. 6: Drug effectiveness on cell proliferation receptor protein expression in HepG2 cells cultured in monolayer.

From: Differential effectiveness of tyrosine kinase inhibitors in 2D/3D culture according to cell differentiation, p53 status and mitochondrial respiration in liver cancer cells

Fig. 6

Effect of Sorafenib, Regorafenib, Lenvatinib, and Cabozantinib in the protein expression of EGFR and c-Met in HepG2 cells cultured in 2D system. Drugs (10µM) were administered at 24h after plating. The expression of tyrosine kinase receptors was assessed 24h after drug administration by SDS–PAGE electrophoresis coupled to western blot procedure as described in “Materials and methods” section. The values were obtained by densitometric analysis of the spots in relation to their loading control in the blots (densitometry, %, fold over control). All results are expressed as mean±SD of independent experiments (n = 3). The level of significance was set at *p ≤ 0.05 and **p ≤ 0.01 in comparison with their corresponding control.

Back to article page