Table 1 Basal oxygen consumption rate (OCR), mitochondrial-dependent ATP generation, and maximal respiration capacity of HepG2, Huh7, Hep3B, SNU423, and SNU449 cell lines cultured in 2D system.

From: Differential effectiveness of tyrosine kinase inhibitors in 2D/3D culture according to cell differentiation, p53 status and mitochondrial respiration in liver cancer cells

Cell lines

OCR

ATP generation

Maximal respiration capacity

HepG2

5.73 ± 0.429a

3.86 ± 1.353a

12.52 ± 0.801b

Huh7

7.04 ± 0.728a

3.45 ± 1.412a

20.26 ± 1.312a

Hep3B

5.50 ± 0.442a

4.52 ± 0.624a

10.52 ± 0.962b,c

SNU423

1.97 ± 0.366b

1.43 ± 0.213b

2.43 ± 0.620d

SNU449

3.46 ± 1.061b

0.45 ± 0.263c

9.18 ± 1.103c

  1. The parameters were measured in cells 48 h after plating and expressed (pmol/min/µg protein) according to MitoStress kit protocol in a XFp Extracellular Flux Analyzer as described in “Materials and methods” section. All results are expressed as mean ± SD of independent experiments (n = 6). The groups with statistically significant differences (p ≤ 0.05) were indicated with different letters (a, b, c, or d).