Fig. 5: 3D organoids recapitulate histopathological features of AM.
From: LGR5+ epithelial tumor stem-like cells generate a 3D-organoid model for ameloblastoma
a A diagram showing the generation of 3D organoids by AM epithelial cells. Ex vivo expanded primary AM epithelial cells or AM-1 cells were transferred to 3D Matrigel and cultured for different days. Scale bars, 20 μm. b 3D-organoid culture of follicular AM epithelial cells for 10 days. Left, H&E staining showed AM epithelial cells arranged into follicular-type organoids. AM organoids recapitulated certain histopathological features of AM, including hyperchromic nuclei cuboidal (ameloblast-like) peripheral cells arranged in a palisading-like pattern and showed reverse polarity. Right: LGR5 was enriched and co-expressed with pan-cytokeratin and activated β-catenin (ABC) in 3D organoids formed by AM epithelial cells as determined by immunofluorescence studies. Scale bars, 20 μm. c 3D-organoid culture of AM-1 (plexiform type) for 10 days. Left: H&E staining showed generated organoids with irregular epithelial islands connected as anastomosing strands that were similar to the histopathological features of plexiform AM. Right, LGR5 was enriched and co-expressed with pan-cytokeratin and activated β-catenin (ABC) in 3D organoids formed by AM epithelial cells as determined by immunofluorescence studies. Scale bars, left upper: 200 μm; left lower and right: 20 μm. All results are representative of three independent experiments.
