Fig. 5: IL-18 is sufficient to induce astrocyte conversion to the A1 phenotype. | Cell Death & Disease

Fig. 5: IL-18 is sufficient to induce astrocyte conversion to the A1 phenotype.

From: Inhibition of the NLRP3-inflammasome prevents cognitive deficits in experimental autoimmune encephalomyelitis mice via the alteration of astrocyte phenotype

Fig. 5

a A schematic diagram of primary astrocytes treatment with IL-18. b qPCR analysis of cultured astrocytes treated with IL-18 (A1 marker: H2-T23, Fkbp5 and ligp1; A2 marker: Emp1, S100A10, and Cd109). c Western blots and densitometric analysis for C3d, p-p65, and p65. The relative expression of C3d normalized to β-actin and the relative expression of p-p65 normalized to p65. d Immunofluorescence for C3d (green) and p65 (red) in cultured astrocytes. The data are the mean ± SEM, n = 6, *P < 0.05, **P < 0.01, ***P < 0.001.

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