Fig. 6: IL-18-induced A1 reactive astrocytes impair hippocampal neurons through the release of C3. | Cell Death & Disease

Fig. 6: IL-18-induced A1 reactive astrocytes impair hippocampal neurons through the release of C3.

From: Inhibition of the NLRP3-inflammasome prevents cognitive deficits in experimental autoimmune encephalomyelitis mice via the alteration of astrocyte phenotype

Fig. 6

a A schematic diagram of hippocampal neurons treatment with different astrocyte conditioned medium (ACM). b–d Representative images and the quantification of immunostaining for synapsin I and PSD95 in cultured neurons treated with control ACM, IL-18 induced ACM or IL-18 induced ACM + SB290157. e Western blots and densitometric analysis for synapsin I, PSD95, and cleaved caspase-3 in cultured neurons. β-actin was used as an internal control. f Representative traces of whole-cell patch clamp evoked AP recordings from cultured neurons. g The quantification of APs evoked with different current injections from 0 to 50 pA with a duration of 200 ms. h Representative traces of whole-cell patch clamp sEPSC recordings from cultured neurons. i Quantification of sEPSC amplitude. j Quantification of sEPSC frequency. The data are the mean ± SEM, n = 6, *P < 0.05, **P < 0.01, ***P < 0.001.

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