Fig. 3: DLEU2 regulates PIK3CD expression, which is correlated with tumor progression and survival time in LSCC patients. | Cell Death & Disease

Fig. 3: DLEU2 regulates PIK3CD expression, which is correlated with tumor progression and survival time in LSCC patients.

From: Long noncoding RNA DLEU2 predicts a poor prognosis and enhances malignant properties in laryngeal squamous cell carcinoma through the miR-30c-5p/PIK3CD/Akt axis

Fig. 3

a, b DLEU2 was overexpressed or knocked down in LSCC cells. Western blot (a) and qRT-PCR (b) analysis were performed to detect PIK3CD expression. β-actin was used as an internal control. c Representative immunohistochemical staining of PIK3CD in normal control, low DLEU2 expression, and high DLEU2 expression samples, respectively (original magnification: ×200; scale bar = 50 µm). d Spearman’s correlation analysis was performed to evaluate the relationship between the mRNA levels of DLEU2 and PIK3CD in 66 LSCC specimens. e The mRNA levels of PIK3CD in 66 LSCC specimens and matched adjacent normal tissues were evaluated by qRT-PCR analysis. f–i Correlations between PIK3CD expression and the tumor stage (f), lymph node metastasis (g), tumor node metastasis (h), and differentiation (i) in LSCC specimens. j The correlation between survival time and PIK3CD expression was analyzed in LSCC patients. **P < 0.01, ***P < 0.001.

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