Fig. 4: BIG1-cKO mice were protected from polymicrobial sepsis in the CLP model.

WT and BIG1-cKO mice were subject to CLP surgery. a The mortality of WT and BIG1-cKO septic mice were assessed daily for up to 10 days. Survival rate were calculated according to the Kaplan–Meier method. Survival analysis was performed using log-rank test (***P < 0.001, n = 10). b Loss of body temperature and weight in WT mice and their littermates BIG1-cKO mice were measured at the indicated time point. Mean ± SEM is presented and analyzed by Student’s t-test (***P < 0.001; n = 6). c The mRNA levels of cytokines in peritoneal macrophages from CLP-treated WT and BIG1-cKO mice were measured at the indicated time point. Mean ± SEM is presented and analyzed by Student’s t-test (**P < 0.01, ***P < 0.001; n = 6). d Serum levels of TNF-α, IL-6, IL-1β, and IL-12 from WT and BIG1 cKO mice were measured by ELISA at the indicated time point. Mean ± SEM is presented and analyzed by Student’s t-test (*P < 0.05, **P < 0.01, ***P < 0.001; n = 6). e, f Liver and lung sections from WT and BIG1 cKO mice were subjected to H&E staining at the indicated time point. The representative images were shown (original magnification, ×400; Scale bars, 50 μm). The inflammatory infiltration score was quantified. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001; n = 6; Student’s t-test.