Fig. 2: Alteration of APN can regulate liver cancer cell metastasis in vitro and in vivo. | Cell Death & Disease

Fig. 2: Alteration of APN can regulate liver cancer cell metastasis in vitro and in vivo.

From: APN-mediated phosphorylation of BCKDK promotes hepatocellular carcinoma metastasis and proliferation via the ERK signaling pathway

Fig. 2

a After CRISPR genome editing, APN was knockout in SK-HEP-1 cells. b After lentivirus transfection, APN was overexpressed in Huh6 cells. c Transwell assays were used to evaluate the migration and invasion of SK-HEP-1 cell after knockout of APN. d Transwell assays were used to evaluate the migration and invasion of Huh6 cell after APN overexpression. Scale bar: 100 μm. The experiments were performed three times. Mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t-test. e Representative bioluminescence imaging of intrahepatic metastatic model derived from splenic implantation with luciferase-labeled SK-Con and SK-KO cells (n = 6 in each group). Representative gross specimens of metastatic lesions in liver, lung, and kidney. Occurance rates of metastasis in different organ metastases are represented by histogram. f Kaplan–Meier plot showing overall survival of mice injected with SK-Con and SK-KO cells via liver (n = 6 in each group). P = 0.0092, log-rank test.

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