Fig. 3: HUWE1 is the DNA-PKcs neddylation E3. | Cell Death & Disease

Fig. 3: HUWE1 is the DNA-PKcs neddylation E3.

From: HUWE1-dependent DNA-PKcs neddylation modulates its autophosphorylation in DNA damage response

Fig. 3

a, b HUWE1 interacts with DNA-PK complex and UBE2M. Immunoprecipitation was performed with DNA-PKcs antibody or Flag-HUWE1, either HUWE1 or DNA-PKcs, Ku70/Ku80, UBE2M was detected by western blotting with indicated antibodies. c HUWE1 depletion abolished DNA-PKcs neddylation. HUWE1 was depleted with specific siRNAs. The depleted cells were harvested and subjected to IP with DNA-PKcs antibody following detection with NEDD8 antibody.

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