Fig. 5: IFN-λ1 suppressed RANKL or LPS-induced osteoclast fusion significantly. | Cell Death & Disease

Fig. 5: IFN-λ1 suppressed RANKL or LPS-induced osteoclast fusion significantly.

From: Dendritic cells-derived interferon-λ1 ameliorated inflammatory bone destruction through inhibiting osteoclastogenesis

Fig. 5: IFN-λ1 suppressed RANKL or LPS-induced osteoclast fusion significantly.The alternative text for this image may have been generated using AI.

a Representative images of FAK staining of RAW264.7 cells treated with RANKL and M-CSF alone or together with the indicated concentrations of IFN-λ1 treatment. F-actin using tetramethylrhodamine-conjugated phalloidin (red), focal contacts using anti-vinculin mAb, and nuclear counterstaining using DAPI (blue). Scale bar = 200 μm. Quantitative analysis of osteoclasts (nucleiå 3) and average osteoclast nuclei number in each field. b Representative images of FAK staining of RAW264.7 cells treated with RANKL and M-CSF alone or together with the indicated concentrations of IFN-λ1 treatment. F-actin using tetramethylrhodamine-conjugated phalloidin (red), focal contacts using anti-vinculin mAb, and nuclear counterstaining using DAPI (blue). Scale bar = 200 μm. Quantitative analysis of osteoclasts (nuclei > 3) and average osteoclast nuclei number in each field. The data in the figures represent the averages ± SD. Significant differences are indicated as *p < 0.05 or **p < 0.01 paired using Student’s t-test unless otherwise specified.

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