Fig. 1: Expression of HSCs activation-related genes and miR-451/miR-185 in activated HSC cell lines. | Cell Death & Disease

Fig. 1: Expression of HSCs activation-related genes and miR-451/miR-185 in activated HSC cell lines.

From: Synergistic antifibrotic effects of miR-451 with miR-185 partly by co-targeting EphB2 on hepatic stellate cells

Fig. 1

a The mRNA levels of Col 1A1, α-SMA and EphB2 were examined in LX-2 cells using RT-qPCR. b The protein expressions of EphB2, MMP2, α-SMA and TIMP2 were analyzed in LX-2 cells using western blotting. c Protein bands in (b) were quantified by ImageJ software. d The mRNA levels of Col 1A1, α-SMA and EphB2 were examined in HSC-T6 cells using RT-qPCR. e The protein expressions of EphB2, MMP2, α-SMA and TIMP2 were analyzed in HSC-T6 cells using western blotting. f Protein bands in (e) were quantified by ImageJ software. g The expression of miR-451/miR-185 was measured in LX-2 cells by RT-qPCR. h The expression of miR-451/miR-185 was measured in HSC-T6 cells by RT-qPCR. Data represent the means ± SEM obtained from triplicate experiments (Student’s t test, *P < 0.05, **P < 0.01, and ***P < 0.001).

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