Fig. 5: MiR-451 and miR-185 synergistically suppress HSCs activation in vitro. | Cell Death & Disease

Fig. 5: MiR-451 and miR-185 synergistically suppress HSCs activation in vitro.

From: Synergistic antifibrotic effects of miR-451 with miR-185 partly by co-targeting EphB2 on hepatic stellate cells

Fig. 5

a RT-qPCR analysis of the mRNA levels of MMP2, α-SMA, and TIMP2 in LX-2 cells transfected with miR-451 mimics, miR-185 mimics, their combinations or control. b, c Western blotting analysis of EphB2 and MMP2, α-SMA, and TIMP2 in LX-2 cells transfected with miR-451 mimics, miR-185 mimics, their combinations or control. d RT-qPCR analysis of the mRNA levels of MMP2, α-SMA, and TIMP2 in HSC-T6 cells transfected with miR-451 mimics, miR-185 mimics, their combinations or control. e, f Western blotting analysis of EphB2, MMP2, α-SMA and TIMP2 in HSC-T6 cells transfected with miR-451 mimics, miR-185 mimics, their combinations or control. Data are shown as the means ± SEM obtained from triplicate experiments (Student’s t test, *P < 0.05, **P < 0.01, and ***P < 0.001).

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